Checkbox disabling an input

I want to make it so when the user clicks the checkbox , the player Two input goes disabled. My problem is that the input remains disabled in both cases, doesn't matter if checkbox is checked or not.

const Initialization = (function() {
  p1 = '';
  p2 = '';

  const playerOne = document.querySelector('#player1')
  const playerTwo = document.querySelector('#player2')
  const checkAI = document.querySelector('#computer')
  const startButton = document.querySelector('#start')

  startButton.addEventListener('click', () => {
    p1 = Player(playerOne.value)
    p2 = Player(playerTwo.value)
  })

  if (checkAI.checked = true) {
    playerTwo.disabled = true;
  } else {
    playerTwo.disabled = false;
  }

  return {
    p1,
    p2,

  }
})();

<label>Computer: <input type="checkbox" id="computer"></label><br/>
<input type="text" id="player1"><br/>
<input type="text" id="player2"><br/>
<input type="button" id="start" value="Start" />