Pyqt5 designer how to run program when check box ticked

So i've created ui and i also have program that is working properly. I need to change boolean value of variable in the exact program after checkbox is ticked and i have four values like that so that means four check buttons, also i have there STOP and START buttons and as they say when i click stop i need to stop the program but not the ui and when i click start i need the program start but already with the boolean values. I've tried it but as soon as i ticked one thing the ui was not responding and the program was running but the user could not tick any other checkboxes. This is the code of the ui.

from PyQt5 import QtCore, QtGui, QtWidgets


class Ui_MainWindow(object):
    def setupUi(self, MainWindow):
        MainWindow.setObjectName("MainWindow")
        MainWindow.resize(541, 367)
        MainWindow.setMinimumSize(QtCore.QSize(541, 367))
        MainWindow.setMaximumSize(QtCore.QSize(541, 367))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 0, 0))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Button, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Base, brush)
        brush = QtGui.QBrush(QtGui.QColor(44, 47, 51))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Window, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 0, 0))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Button, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Base, brush)
        brush = QtGui.QBrush(QtGui.QColor(44, 47, 51))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Window, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 0, 0))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Button, brush)
        brush = QtGui.QBrush(QtGui.QColor(44, 47, 51))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Base, brush)
        brush = QtGui.QBrush(QtGui.QColor(44, 47, 51))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Window, brush)
        MainWindow.setPalette(palette)
        font = QtGui.QFont()
        font.setUnderline(True)
        MainWindow.setFont(font)
        MainWindow.setAutoFillBackground(True)
        self.centralwidget = QtWidgets.QWidget(MainWindow)
        self.centralwidget.setObjectName("centralwidget")
        self.Bludiste = QtWidgets.QCheckBox(self.centralwidget)
        self.Bludiste.setGeometry(QtCore.QRect(50, 10, 191, 71))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(0, 0, 0, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.PlaceholderText, brush)
        self.Bludiste.setPalette(palette)
        font = QtGui.QFont()
        font.setFamily("Calibri")
        font.setPointSize(14)
        font.setItalic(True)
        font.setUnderline(True)
        self.Bludiste.setFont(font)
        self.Bludiste.setObjectName("Bludiste")
        self.line = QtWidgets.QFrame(self.centralwidget)
        self.line.setGeometry(QtCore.QRect(20, 10, 20, 311))
        self.line.setFrameShape(QtWidgets.QFrame.VLine)
        self.line.setFrameShadow(QtWidgets.QFrame.Sunken)
        self.line.setObjectName("line")
        self.line_2 = QtWidgets.QFrame(self.centralwidget)
        self.line_2.setGeometry(QtCore.QRect(30, 310, 481, 20))
        self.line_2.setFrameShape(QtWidgets.QFrame.HLine)
        self.line_2.setFrameShadow(QtWidgets.QFrame.Sunken)
        self.line_2.setObjectName("line_2")
        self.line_3 = QtWidgets.QFrame(self.centralwidget)
        self.line_3.setGeometry(QtCore.QRect(500, 10, 20, 311))
        self.line_3.setFrameShape(QtWidgets.QFrame.VLine)
        self.line_3.setFrameShadow(QtWidgets.QFrame.Sunken)
        self.line_3.setObjectName("line_3")
        self.line_4 = QtWidgets.QFrame(self.centralwidget)
        self.line_4.setGeometry(QtCore.QRect(30, 0, 481, 16))
        self.line_4.setFrameShape(QtWidgets.QFrame.HLine)
        self.line_4.setFrameShadow(QtWidgets.QFrame.Sunken)
        self.line_4.setObjectName("line_4")
        self.Stop = QtWidgets.QToolButton(self.centralwidget)
        self.Stop.setGeometry(QtCore.QRect(110, 260, 151, 51))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 0, 0))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 0, 0))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        self.Stop.setPalette(palette)
        font = QtGui.QFont()
        font.setPointSize(13)
        font.setItalic(False)
        font.setUnderline(True)
        font.setStrikeOut(False)
        self.Stop.setFont(font)
        self.Stop.setObjectName("Stop")
        self.start = QtWidgets.QToolButton(self.centralwidget)
        self.start.setGeometry(QtCore.QRect(310, 260, 151, 51))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(53, 167, 12))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(53, 167, 12))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        self.start.setPalette(palette)
        font = QtGui.QFont()
        font.setPointSize(13)
        font.setItalic(False)
        font.setUnderline(True)
        font.setStrikeOut(False)
        self.start.setFont(font)
        self.start.setObjectName("start")
        self.Vyprava = QtWidgets.QCheckBox(self.centralwidget)
        self.Vyprava.setGeometry(QtCore.QRect(50, 70, 191, 71))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(0, 0, 0, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.PlaceholderText, brush)
        self.Vyprava.setPalette(palette)
        font = QtGui.QFont()
        font.setFamily("Calibri")
        font.setPointSize(14)
        font.setItalic(True)
        font.setUnderline(True)
        self.Vyprava.setFont(font)
        self.Vyprava.setObjectName("Vyprava")
        self.Circusturma = QtWidgets.QCheckBox(self.centralwidget)
        self.Circusturma.setGeometry(QtCore.QRect(260, 10, 211, 71))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(0, 0, 0, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.PlaceholderText, brush)
        self.Circusturma.setPalette(palette)
        font = QtGui.QFont()
        font.setFamily("Calibri")
        font.setPointSize(14)
        font.setItalic(True)
        font.setUnderline(True)
        self.Circusturma.setFont(font)
        self.Circusturma.setObjectName("Circusturma")
        self.Arena = QtWidgets.QCheckBox(self.centralwidget)
        self.Arena.setGeometry(QtCore.QRect(260, 70, 211, 71))
        palette = QtGui.QPalette()
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Active, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(255, 255, 255, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Inactive, QtGui.QPalette.PlaceholderText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.WindowText, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.Text, brush)
        brush = QtGui.QBrush(QtGui.QColor(120, 120, 120))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.ButtonText, brush)
        brush = QtGui.QBrush(QtGui.QColor(0, 0, 0, 128))
        brush.setStyle(QtCore.Qt.SolidPattern)
        palette.setBrush(QtGui.QPalette.Disabled, QtGui.QPalette.PlaceholderText, brush)
        self.Arena.setPalette(palette)
        font = QtGui.QFont()
        font.setFamily("Calibri")
        font.setPointSize(14)
        font.setItalic(True)
        font.setUnderline(True)
        self.Arena.setFont(font)
        self.Arena.setObjectName("Arena")
        MainWindow.setCentralWidget(self.centralwidget)
        self.statusbar = QtWidgets.QStatusBar(MainWindow)
        self.statusbar.setObjectName("statusbar")
        MainWindow.setStatusBar(self.statusbar)

        self.retranslateUi(MainWindow)
        QtCore.QMetaObject.connectSlotsByName(MainWindow)

    def retranslateUi(self, MainWindow):
        _translate = QtCore.QCoreApplication.translate
        MainWindow.setWindowTitle(_translate("MainWindow", "Gladiatus-Bot"))
        self.Bludiste.setText(_translate("MainWindow", "Chodit do bludiště"))
        self.Stop.setText(_translate("MainWindow", "Stop"))
        self.start.setText(_translate("MainWindow", "Start"))
        self.Vyprava.setText(_translate("MainWindow", "Chodit na výpravy"))
        self.Circusturma.setText(_translate("MainWindow", "Chodit do Circus Turma"))
        self.Arena.setText(_translate("MainWindow", "Chodit do Arény"))


if __name__ == "__main__":
    import sys
    app = QtWidgets.QApplication(sys.argv)
    MainWindow = QtWidgets.QMainWindow()
    ui = Ui_MainWindow()
    ui.setupUi(MainWindow)
    MainWindow.show()
    sys.exit(app.exec_()) 

I would be so grateful for any help or hints. Thank you!

Also for the more interested this is the code. The parts u care about are cutted out for you because the program has 5 times over the limit of characters

import time
import pyautogui
import os
import subprocess

health_status = 0
slots_taken = 0
k = 0
d = 0
o = ""
y = ""
muk = 0
mut = 0
mup = 0
mub = 0


def do_it():
    global d, muk, mut, mup, mub, play
    global o, y
    global k
    global health_status
    play = ""

    while play == "":
        play = pyautogui.locateCenterOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\play.jpg',
                                              confidence=0.95, grayscale=False)

        expedition_loc = ""
        lost_v2 = ""
        circus_turma = ""
        arena = ""
        expedition_permission = True
        lost_v2_permission = True
        circus_turma_permission = True
        arena_permission = True
        while expedition_loc == 


while True:
    x = ""
    while x is not None:
        x = pyautogui.locateOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\pidi.jpg', confidence=0.9,
                                     minSearchTime=6)
        do_it()
    else:
        xz = pyautogui.locateCenterOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\x.jpg', confidence=0.701)
        while xz is not None:
            xz = pyautogui.locateCenterOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\x.jpg',
                                                confidence=0.701)
            pyautogui.click(xz)
        else:
            time.sleep(2)
            po = pyautogui.locateCenterOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\XX.jpg', confidence=0.5)
            if po is not None:
                pyautogui.click(1899, 15)
            else:
                ""
            time.sleep(1)
            commmand = '"C:\\Users\\Igor\\AppData\\Local\\Programs\\Opera\\launcher.exe"' \
                       'https://lobby.gladiatus.gameforge.com/cs_CZ/hub'
            subprocess.Popen(commmand)
            time.sleep(5)
            play = pyautogui.locateCenterOnScreen('C:\\Users\\Igor\\PycharmProjects\\Udemy\\done\\play.jpg',
                                                  confidence=0.8, grayscale=False)
            if play is not None:
                print("Play button was found")
                pyautogui.click(play, duration=0.2)
                time.sleep(1)
                pyautogui.click(1404, 350, duration=0.2)
                time.sleep(5)
                pyautogui.click(222, 15)