ASP.NET CheckBox not checked on postback without weird hack

I have a GridView with a checkbox column. On clicking a button, all rows with the checkbox checked should be removed. I somehow stumbled upon a strange and hacky solution, and I have no idea why it works. I already searched through related SO questions already.

Related code:

Protected Sub Page_Init(ByVal sender As Object, ByVal e As EventArgs) Handles Me.Init
    ' I have no idea why this is needed for the checkboxes to work...
    Dim x = imageGridView.Rows
End Sub


Protected Sub RemoveButton_Click(ByVal sender As Object, ByVal e As EventArgs) Handles removeButton.Click

    For Each row As GridViewRow In imageGridView.Rows
        Dim selectCheckBox As CheckBox = DirectCast(row.Cells(0).FindControl("selectCheckBox"), CheckBox)
        If selectCheckBox.Checked Then
            Dim fileName As String = row.Cells(1).Text
            ImageList.Remove(ImageList.FindLast(Function(r) r.FileName = fileName))
        End If
    Next
    imageGridView.DataSource = ImageList
    imageGridView.DataBind()
End Sub

Aspx:

<asp:GridView ID="imageGridView" runat="server">
    <Columns>
        <asp:TemplateField>
            <ItemTemplate>
                <asp:CheckBox ID="selectCheckBox" runat="server" />
            </ItemTemplate>
        </asp:TemplateField>
    </Columns>
</asp:GridView>

The line Dim x = imageGridView.Rows is needed for the rows to be removed. I found this after trying my RemoveButton_Click code in the Page_Init sub, then removing code until it didn't work anymore. Dim x = imageGridView is not enough, and it doesn't work to do the same thing in Page_Load.

My checkboxes are never disabled.

So, simply put, why is it necessary for me to reference imageGridView.Rows in the Page_Init for my code to work?